Rachel Boston was born and raised in Signal Mountain, Tennessee. At 17, she moved to New York City to pursue an acting career. At 19, Boston drove to Los Angeles and began filming NBC's award-winning Television Series, American Dreams (2002).

For her starring role as Mindy in the independent feature The Pill (2011), Boston was honored with the Stargazer Award for the most talented emerging actress at the Gen Art Film Festival in New York City, the Best Actress Award from the San Diego Film Festival, and the Emerging Artist Award from the Big Apple Film Festival. Filmed in Manhattan, "The Pill", also took home the Gen Art Film Festival Audience Award.

She previously appeared, with Joseph Gordon-Levitt and Zooey Deschanel, in the Golden Globe? nominated film, (500) Days of Summer (2009), which premiered at the Sundance Film Festival and went on to win an Independent Spirit Award for Best Screenplay. She also co-starred, alongside Matthew McConaughey and Jennifer Garner, in the romantic comedy, Ghosts of Girlfriends Past (2009).

Boston starred as Ingrid Beauchamp in the television series, Witches of East End (2013). Previously, Boston starred on the USA series In Plain Sight (2008) and the CBS series The Ex List (2008). Some of her guest-starring appearances include Grey's Anatomy (2005), Curb Your Enthusiasm (2000), ER (1994), NCIS: Naval Criminal Investigative Service (2003), Rules of Engagement (2007) and Mad Love (2011).